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anti rabbit igg h l hrp conjugate  (Bio-Rad)


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    Structured Review

    Bio-Rad anti rabbit igg h l hrp conjugate
    Anti Rabbit Igg H L Hrp Conjugate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 7038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+rabbit+hrp/Goat+Anti-Rabbit+IgG+(H+%2B+L)-HRP+Conjugate/pmc13156693-7-0-7
    Average 99 stars, based on 7038 article reviews
    anti rabbit igg h l hrp conjugate - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Developing a Multimodal miR-15a Mimic to Overcome PARP Inhibitor Resistance in Epithelial Ovarian Cancer
    Article Snippet: Primary antibodies were diluted in 5% milk (Bio-Rad) in TBST. .. After staining with primary antibodies, proteins were probed with either secondary antibodies, goat anti-mouse-HRP (Bio-Rad, 1:5000) or goat anti-rabbit-HRP (Bio-Rad, 1:5000), depending on the used primary antibody. .. Protein bands were visualized using an LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific).

    Incubation:

    Article Title: Estrogen deficiency induces pelvic floor muscle atrophy via ERα/GLUT4 pathway
    Article Snippet: The following primary antibodies were used: GLUT4 (66846–1-Ig, 1:2000, Proteintech, Wuhan, China), RAC1 (66122–1-Ig, 1:3000, Proteintech), TFAM (22586–1-AP, 1:5000, Proteintech), PGC1α (66369–1-Ig, 1:5000, Proteintech), β-actin (66009–1-Ig, 1:20000, Proteintech), MyHC (ab37484, 1:1000, Abcam, Cambridge, UK), ERα ( GB111843 –100, 1:1000, Servicebio) and ERβ ( GB115454 –100, 1:1000, Servicebio). .. Subsequently, membranes were washed with TBST, incubated with goat anti-mouse HRP-conjugated secondary antibody (SA00001–1, 1:5000, Proteintech) or goat anti-rabbit HRP-conjugated secondary antibody (SA00001–2, 1:5000, Proteintech) for 1 hour at room temperature, and visualized using a Bio-Rad ChemiDoc XRS + Gel Imaging System. .. Band intensities were quantified with ImageJ software and normalized to the corresponding β‐actin loading control.

    Article Title: Development of gemcitabine-modified miR-15a as a novel, multimodal treatment strategy to overcome 5-FU and oxaliplatin resistance in colorectal cancer
    Article Snippet: Proteins were probed with rabbit anti-WEE1 (Cell Signaling, 13084, 1:1,000), mouse anti-CHK1 (Cell Signaling, 2360, 1:1,000), rabbit anti-BMI1 (Cell Signaling, 6964, 1:1,000), rabbit anti-YAP1 (Cell Signaling, 4912, 1:1,000), rabbit anti-CCND1 (Cell Signaling, 55506, 1:1,000), rabbit anti-p53 (Cell Signaling, 9282, 1:1,000), mouse anti-p21 (Cell Signaling, 2946, 1:2,000), rabbit anti-CCNB1 (Cell Signaling, 12231, 1:1,000), rabbit anti-CCNA2 (Cell Signaling, 67955, 1:1,000), rabbit anti-Phospho-Histone H2A.X (γH2AX) (Cell Signaling, 2577, 1:1,000), mouse anti-thymidylate synthase (Sigma Aldrich, MAB4130, 1:1,000), rabbit anti-BAK (Cell Signaling, 12105, 1:1,000), rabbit anti-PARP (Cell Signaling, 9532, 1:1,000), rabbit anti-cleaved PARP (Cell Signaling, 5625, 1:1,000), rabbit anti-caspase-7 (Cell Signaling, 12827, 1:1,000), rabbit anti-cleaved caspase-7 (Cell Signaling, 8438, 1:1,000) and mouse anti-β-actin (Merck, A5441, 1:10,000). .. After probing with primary antibodies, which were diluted in 5% milk (Bio-Rad, Hercules, CA, USA) made in TBST, the proteins were incubated with secondary antibodies, either goat anti-rabbit-HRP (Bio-Rad, 1721019, 1:5,000) or goat anti-mouse-HRP (Bio-Rad, 1706516, 1:5,000), depending on the corresponding primary antibody. .. The protein bands were then visualized on the LI-COR Biosciences Odyssey Fc imaging system, after incubating with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA, USA).

    Imaging:

    Article Title: Estrogen deficiency induces pelvic floor muscle atrophy via ERα/GLUT4 pathway
    Article Snippet: The following primary antibodies were used: GLUT4 (66846–1-Ig, 1:2000, Proteintech, Wuhan, China), RAC1 (66122–1-Ig, 1:3000, Proteintech), TFAM (22586–1-AP, 1:5000, Proteintech), PGC1α (66369–1-Ig, 1:5000, Proteintech), β-actin (66009–1-Ig, 1:20000, Proteintech), MyHC (ab37484, 1:1000, Abcam, Cambridge, UK), ERα ( GB111843 –100, 1:1000, Servicebio) and ERβ ( GB115454 –100, 1:1000, Servicebio). .. Subsequently, membranes were washed with TBST, incubated with goat anti-mouse HRP-conjugated secondary antibody (SA00001–1, 1:5000, Proteintech) or goat anti-rabbit HRP-conjugated secondary antibody (SA00001–2, 1:5000, Proteintech) for 1 hour at room temperature, and visualized using a Bio-Rad ChemiDoc XRS + Gel Imaging System. .. Band intensities were quantified with ImageJ software and normalized to the corresponding β‐actin loading control.



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